赵健
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赵健 


博士教授

生物化学与分子生物学专业:博士生导师/硕士生导师

生物工程与技术专业:博士生导师/硕士生导师

生物工程领域:工程硕士导师




科研方面:主要主持科研项目有国家自然科学基金(连续主持两项);国家十一五支撑计划项目:苦瓜降血糖活性研究;国家“863”项目子课题-生物催化合成芳香手性醇基内酰胺研究以及果蔬洗涤用有机磷水解酶研制;浙江省重大新药创制:特异性抗肿瘤生物制品一类新药的合作研发及药企抗肿瘤药物研究项目等;

教学方面:承担本科生,研究生课程教学包括分子生物学,酶学教学等,主编或参编研究生及本科生酶学级酶工程教材10余部,获研究生、本科生教学成果奖10余次,发表论文40余篇, 申请专利10余项。


研究领域与课题内容:
1
特异靶向性抗肿瘤药物的研究与开发

采用基因工程技术,将专一性抗肿瘤多肽分子基因进行克隆、定点突变,转入蛋白表达体系进行大规模表达。研究重组蛋白分子的抗肿瘤特性和机理,进行新药申报的相关体外、体内的临床前新药研究工作。

2 药物传送途径及靶向性分子机理研究

    对外源性生物活性分子体内的跨膜运输工具,特别是人源性穿膜肽进行其跨膜转运机理、转运特性、转运靶向性及转运后去向等从分子和细胞水平进行理论与应用研究。研究、开发高效及新型穿膜肽,用于药物的体内靶向性运输,提高药物的安全性和有效性。

3 .酶工程:酶的改造、酶的结构与功能,工业化应用研究

从多种微生物中筛选、克隆在医药工业、食品工业、能源工业等领域有应用前景的各种新型酶类基因,针对其应用进行酶的结构性质研究与改造,以适用与工业化应用开发。目前进行的相关酶类有:脂肪酶属(用于药物中间体的手性拆分和定向转化),肝素酶属(用于肝素的生物加工和分析)等。

 

代表性部分论著:

1. Analysis of Triterpenoid Saponins Reveals Insights into Structural Features Associated with Potent Protein Drug Enhancement Effects.

Cao, Xue-Wei; Wang, Fu-Jun; Liew, Oi-Wah; Lu, Ye-Zhou; Zhao, Jian*

Molecular Pharmaceutics, 2020, 17(2): 683-694.

             

2. Effective Therapeutic Drug Delivery by GALA3, an Endosomal Escape Peptide with Reduced Hydrophobicity.

Li, Chen; Cao, Xue-Wei; Zhao, Jian*; Wang, Fu-Jun*

Journal of Membrane Biology, 2020.

             

3. Screening and characterization of a novel high-efficiency tumor-homing cell-penetrating peptide from the buffalo cathelicidin family

Xu, Yuan Yuan; Cao, Xue Wei; Fu, Long Yun; Zhang, Tao Zhu; Wang, Fu Jun*; Zhao, Jian*

Journal of Peptide Science, 2019, 25(9): 0-UNSP e3201.

 

4. Enhanced anticancer effect of MAP30-S3 by cyclosproin A through endosomal escape.

Song; Zhen-Tao; Zhang; Leshuai W; Fan; Li-Qiang; Kong; Song, Zhen-Tao; Zhang, Leshuai W; Fan, Li-Qiang; Kong, Jing-Wen; Mao, Jun-Hua; Zhao, Jian*; *Wang, Fu-Jun

Anti-Cancer Drugs, 2018, 29(8): 736-747.

 

5. Structure optimisation to improve the delivery efficiency and cell selectivity of a

  tumour-targeting cell-penetrating peptide.

  Cao; Xue-Wei; Yang; Xu-Zhong; Du; Xuan; Fu; Long-Yun; Cao, Xue-Wei; Yang, Xu-Zhong;

  Du, Xuan; Fu, Long-Yun; Zhang, Tao-Zhu; Shan, Han-Wen; Zhao, Jian*; Wang, Fu-Jun

  Journal of Drug Targeting, 26(9), pp 777-792, 2018-1-16

 

6. Enhanced anticancer effect of MAP30-S3 by cyclosproin A through endosomal escape.

  Song; Zhen-Tao; Zhang; Leshuai W; Fan; Li-Qiang; Kong; Song, Zhen-Tao; Zhang, Leshuai W;  

  Fan, Li-Qiang; Kong, Jing-Wen; Mao, Jun-Hua; Zhao, Jian*;*Wang, Fu-Jun

   Anti-Cancer Drugs, 29(8), pp 736-747, 2018-6-14

 

7. A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells.

   Lin B, Yang XZ, Cao XW, Zhang TZ, Wang FJ, Zhao, Jian*. Biotechnol Lett. 2017

   Jan;39(1):71-78  2016 Oct 6.

 

8.  Effectively enhancing cytotoxic and apoptotic effects of alpha-momorcharin by integrating a

   heparin-binding peptide.  Tan MJ, Cao XW, Li PF, Zhai YZ, Zhou Y, Liu YJ, Zhao J*, Wang

   FJ. Biotechnol Appl Biochem. 2017 Nov;64(6):918-926. 2017 Apr 27.


9. Qiang Lv; Xu-Zhong Yang; Long-Yun Fu; Yv-Ting Lu; Yan-Hua Lu; Zhao J*; Fu-Jun Wang*, Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity, Protein Expression and Purification, 2015:111,9-17

10. Nguyen, Long The; Yang, Xu-Zhong; Du, Xuan; Wang, Jia-Wei; Zhang, Rui; Zhao J*;   Wang, Fu-Jun*; Dong, Yang; Li, Peng-Fei, Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR., Amino Acids, 2015: 47,997–1006.

11. Zhang, Rui; Yang, Xu-Zhong; Wang, Jia-Wei; Fu, Long-Yun; Zhao J*; Wang, Fu-Jun*, Evaluating the translocation properties of a new nuclear targeted penetrating peptide using two fluorescent markers., J Drug Target, 2015:1, 1-9.

12. Tian, Miao; Zeng, Xiang-Qing; Song, Huan-Lei; Hu, Shan-Xin; Wang, Fu-Jun*; Zhao J*;   Hu, Zhi-Bi, Molecular diversity and hypoglycemic polypeptide-P content of Momordica charantia in different accessions and different seasons., J Sci Food Agric, 95(6), pp 1328-1335, 2015.

13. Luo, Xiao-Jing; Kong, Xu-Dong; Zhao J Chen, Qi; Zhou, Jiahai; Xu J-H*. Switching a Newly Discovered Lactonase into an Efficient and Thermostable Phosphotriesterase by Simple Double Mutations His250Ile/Ile263Trp, Biotechnology and Bioengineering, 111(10), pp 1920-1930, 2014/10.

14. JJ Shangguan, L Fan, X Ju, Q Zhu, FJ Wang, Zhao J*, Xu J-H*. Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus. Appl Biochem Biotechnol. 2012;168:1820–1833

15. Zhao J*, Gao P, Xiao W, Fan LQ, Wang FJ, Li SX, Liu JW. A novel human derived cell-penetrating peptide in drug delivery. Mol Biol Rep. 2011 38:2649–2656

16. Wang BL, Zhang WJ, Zhao J*, Wang FJ* et al. Gene cloning and expression of a novel hypoglycaemic peptide from Momordica. charantia. J Sci Food Agric. 2011; 91: 2443–2448

17. Shangguan JJ , Liu YQ, Wang FJ, Zhao J*, Fan LQ, Li SX, Xu J-H*. Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity Appl Biochem Biotechnol. Appl Biochem Biotechnol. 2011;165:949–962

18. Wang Y, Zhao J*, Xu JH*, Fan LQ, Li SX, Zhao LL, Mao XB. Significantly Improved Expression and Biochemical Properties of ecombinant Serratia marcescens Lipase as Robust Biocatalyst for  Kinetic Resolution of Chiral Ester. Appl Biochem Biotechnol, 2010 162(8):2387-99.

联系方式:Tel642522556425225664252257  E.mailzhaojian@ecust.edu.cn