高蓓
发布人:2020十大正规网赌网址网站管理员  发布时间:2018-01-17   动态浏览次数:9262

  高蓓

 

生物化工专业:硕士生导师

生物工程专业:硕士生导师

生物与医药工程领域:工程硕士生导师



高蓓,全球十大网赌排行app副教授、硕士生导师。2010年获华东理工大学生物化工专业工学博士学位。长期致力于合成生物学、分子生物学、生物催化与生物转化等领域相关研究工作。作为负责人先后主持国家科技部“863”计划、国家自然科学基金、上海市自然科学基金、中央高校基本科研业务费探索基金等多项国家及省部级课题,并与恒瑞医药股份有限公司、一鸣生物股份有限公司等多家企业进行合作。在“Metabolic Engineering”“Scientific Reports”“Journal of Biotechnology”“Journal of Agricultural and Food Chemistry”等本领域重点期刊上发表论文多篇,授权专利多项。

研究方向:

1,微生物合成生物学:结合组学、代谢工程和合成生物学技术,开发高效能微生物,实现天然产物的高效发酵生产。

2,生物催化剂的高效创制:通过酶资源开发、催化机理解析、分子理性设计改造等策略,创制高效生物催化剂,开发功能食品的绿色合成工艺。

主持或参加的科研项目或基金:

1,  国家自然科学基金面上项目,317720072018-2021,项目负责人。

2,  企业横向课题,热启动DNA聚合酶的开发,2019-2021,项目负责人。

3,  国家高技术发展(863)计划,2013AA1021092013-2017,课题负责人。

4,  上海市自然科学基金项目,16ZR14495002016-2019,项目负责人。

5,  浙江省近岸水域生物资源开发与保护重点实验室开放基金项目,J2015001 2015-2017,项目负责人。

6,  国家自然科学基金青年基金项目,312012962013-2015,项目负责人。

7,  中央直属高校基本科研业务费专项基金探索研究基金,WF1414038 2015-2016,项目负责人。

8,  中央直属高校基本科研业务费专项基金探索研究基金,WF1114022 2012-2013,项目负责人。

9,  企业横向课题,溶菌酶生产菌种和技术开发,2020-2023,课题骨干成员。

10, 国家自然科学基金面上项目,C050203- 315707952016-2019, 课题骨干成员。

11, 国家高技术发展(863)计划,2012AA0204032012-2015,课题骨干成员。

12, 华东理工大学交叉学科与重大项目培育基金,2012-2016,课题骨干成员。

13, 苏恒瑞医药股份有限公司横向课题,胰蛋白酶、羧肽酶B酶制剂的开发,2012-2014,课题骨干成员。

教学:

1,本科生必修课《生物化学》(主讲)

2,本科生必修课《发酵综合实验》(主讲)

3,本科生选修课《食品酶学》(主讲)

代表性论文(*通讯作者):  

1 . Tian Li, Guosong Liu, Wei Zhou, Min Jiang, Yuhong Ren, Xinyi Tao, Min Liu, Ming Zhao, Fengqing Wang, Bei Gao, Dongzhi Wei. Engineering ethanol utilization to overproduce squalene in Saccharomyces cerevisiae. Journal of Agriculture and Food Chemistry. 2020, 68: 2132-2138.

2 . Guo-Song Liu, Tian Li, Wei Zhou, Min Jiang, Xin-Yi Tao, Min Liu, Ming Zhao, Yu-Hong Ren, Bei Gao, Feng-Qing Wang, Dong-Zhi Wei. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction. Metabolic Engineering. 2020, 57:151-161.

3  . Bei Gao, Lei He, Dongzhi Wei, Lujia Zhang*.Identification and magnetic immobilization of a pyrophilous aspartic protease from Antarctic psychrophilic fungus. Journal of Food Biochemistry. 2018, e12691.

4  . Bei Gao, Shuiqin Jiang, Liuzhu Wang, Lujia Zhang*, Dongzhi Wei*. Energy and conformation determine the enantioselectivity of enzyme. Biochemical Engineering Journal, 2018, 129: 106-112.

5.  Zhiqiang Yao, Shuiqin Jiang, Lujia Zhang, Bei Gao, Xiao He, John Z. H. Zhang, Dongzhi Wei. Crius: A novel fragment-based algorithm of de novo substrate prediction for enzymes. Protein Science. 2018, 27: 1526-1534.

6 . Min Jiang, Lujia Zhang, Fengqing Wang, Jie Zhang, Guosong Liu, Bei Gao*, Dongzhi Wei*. Novel application of magnetic protein: Convenient one-step purification and immobilization of proteins. Scientific Reports. 2017, 7: 13329..

7 . Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao*, Dongzhi Wei. Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization. BMC Biotechnology. 2017, 17:22.

8 . Shuiqin Jiang, Lujia Zhang*, Zhiqiang Yao, Bei Gao, Hualei Wang, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science and Technology. 2017, 7(5):1122-1128.

9 . Shuiqin Jiang, Lujia Zhang*, Dongbin Cui, Zhiqiang Yao, Bei Gao, Jinping Lin, Dongzhi Wei*. The Important Role of Halogen Bond in Substrate Selectivity of Enzymatic Catalysis. Scientific Reports. 2016, 6: 34750.

10Zhiqiang Yao, Lujia Zhang*, Bei Gao, Dongbing Cui, Fengqing Wang, Xiao He, Dongzhi Wei*. A semiautomated structure-based method to predict substrates of enzymes via molecular docking: A case study with Candida antarctica lipase B. Journal of Chemical Information and Modeling. 2016, 56: 1979-1994.

11Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang*, Dongzhi Wei*. A novel esterase from a marine mud metagenomics library for biocatalytic synthesis of short chain flavor esters. Microbial Cell Factories. 2016, 15: 41-52

12 .  Bei Gao, Youzhi Mao, Lujia Zhang*, Lei He, Dongzhi Wei. A novel saccharifying α-amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterizationStarch/Stärke. 2015, 67(1): 1-9.

13 . Youzhi Mao, Yanchen Yin, Lujia Zhang, Siti Aisyah Alias, Bei Gao*, Dongzhi Wei. Development of a novel Aspergillus uracil deficient expression system and its application in expressing a cold-adapted α-amylase gene from Antarctic fungi Geomyces pannorum. Process Biochemistry. 2015, 50: 1581-1590

14 . Yanchen Yin, Youzhi Mao, Xiaolie Yin, Bei Gao*, Dongzhi Wei. Construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in Aspergillus oryzaeJournal of Microbiology and Biotechnology. 2015, 25(7): 988-998.

15 . Dongbing Cui, Lujia Zhang*, Shuiqin Jiang, Zhiqiang Yao, Bei Gao, Jinping Lin, Y. Adam Yuan, Dongzhi Wei*. A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H). FEBS Journal. 2015, 282(12): 2339-2351.

16.  Zhenggui He, Lujia Zhang, Youzhi Mao, Jingchao Gu, Qi Pan, Sixing Zhou, Bei Gao*, Dongzhi Wei*. Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with α-amylase in Pichia pastoris. BMC Biotechnology. 2014, 14:114

17Zhenggui He, Yanchen Yin, Youzhi Mao, Sixing Zhou, Qi Pan, Dongzhi Wei, Bei Gao*. Cloning and high-level expression of thermostable α-amylase gene from Rhizomucor pusillus. Journal of Southern Agriculture. 2014, 45(2): 165-172

18Lujia Zhang, Xiaomang Tang, Dongbing Cui, Zhiqiang Yao, Bei Gao, Shuiqin Jiang, Bo Yin, Y. Adam Yuan*, Dongzhi Wei*. A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107. Protein Science. 2014, 23:110-116.

19Lujia Zhang, Bei Gao, Zuanning YuanXiao HeY. Adam YuanJohn Z.H. Zhang*, Dongzhi Wei*. Structure, mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way. Biochimica et Biophysica Acta (BBA) -Proteins and Proteomics. 2014, 1844:1183-1192.

20Xiangping Wu, Pengyong You, Erzheng Su*, Jingjing Xu, Bei Gao, Dongzhi Wei*. In vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli. BMC Biotechnology, 2012, 12: 58-64.

21Bei Gao, Tao Xu, Jinping Lin, Lujia Zhang, Zhengbing Jiang, Dongzhi Wei*. Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation. Journal of Molecular Catalysis B: Enzymatic, 2011, 68: 286–291.

22Huiting Song, Lichao Zhou, Lujia Zhang, Bei Gao, Dongzhi Wei, Yaling Shen, Rui Wang, Catherine Madzak, Zhengbing Jiang*, Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily wastewaters. Journal of Molecular Catalysis B: Enzymatic. 2011, 71:166-170.

23Tao Xu, Bei Gao, Lujia Zhang, Jingpin Lin, Xuedong Wang*, Dongzhi Wei*. Template-based modeling of a psychrophilic lipase: conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 2010, 1804: 2183-2190.

24 . Bei Gao, Erzheng Su, Jinping Lin, Dongzhi Wei*.Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production. Journal of Biotechnology, 2009, 139(2):169-175.

25 . Bei GaoZhengbing Jiang, Ren Ren, Dongzhi Wei*Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparision with the LipB52 displayed on Saccharomyces cereisiae cell surfaceBMC Biotechnology, 2008, 8:4: 1-7.

发明专利:

1、  高蓓,魏东芝,张鲁嘉,王风清,江敏,刘国松。一种酶固定化方法及其应用。授权专利号:ZL201710444356.8

2、  魏东芝,高蓓,贺磊,张鲁嘉。一种α-淀粉酶及其编码基因与应用。专利授权号:ZL201610052870.2

3、  高蓓,魏东芝,贺磊,张鲁嘉。一种天冬氨酸类蛋白酶及其编码基因与应用。专利授权号:ZL 201610052686.8

4、  魏东芝,高蓓,毛佑志。一种用于在米曲霉细胞分泌表达外源蛋白的表达设备及其米曲霉基因工程菌。授权专利号:ZL201410260946.1 

5、  高蓓,魏东芝,毛佑志。一种来自真菌的低温α-淀粉酶及其编码基因与应用。专利公开号:201410261362.6

6、  高蓓,魏东芝,毛佑志。一种α-淀粉酶及其编码基因与应用。专利公开号:201410443499.3 

7、  魏东芝,高蓓,何正贵。一种共表达葡萄糖淀粉酶和α-淀粉酶的重组毕赤酵母菌株及其构建方法,以及混合酶制剂。授权专利号:ZL201310033964.1

8、  高蓓,魏东芝,何正贵。一种耐热葡萄糖淀粉酶及其编码基因与应用。授权专利号:ZL201310016408.3

9、  魏东芝,高蓓,马昱澍,苏二正。脂肪酶基因以及工程菌和脂肪酶菌株。授权专利号:ZL200810033414.9

联系方式:

电话:021-6425180318019078882

传真:021-64250068

邮箱:gaobei@ecust.edu.cn

通讯地址:生物工程学院生物反应器国家重点实验十八楼707

上海市徐汇区梅陇路130华东理工大学

邮编:200237