林金萍
发布人:2020十大正规网赌网址网站管理员  发布时间:2016-10-19   动态浏览次数:5458

  

林金萍

生物化学与分子生物学专业:硕士生导师

生物化工专业:硕士生导师

生物与医药工程领域:工程硕士生导师



个人简介:

林金萍,全球十大网赌排行app副研究员、硕士生导师。现任中国轻工业生物催化与智能制造重点实验室副主任。1997年和2000年分别于南昌大学和无锡轻工大学获学士和硕士学位,2006年毕业于华东理工大学,获生物化工专业博士学位。

长期从事生物催化与酶工程、合成生物学等领域相关研究工作。主持和参与国家自然科学基金、“973”课题、重大专项以及企业横向项目18项。发表学术论文60余篇。申请发明专利 12项,其中授权9项。获得国家技术发明二等奖1项、上海市技术发明一等奖1项和上海市科技进步奖一等奖2项。获得国家新药临床批件2项、生产批文2项和新药证书1项,实现成果转让3项。2016年入选上海市青年科技英才。


主要研究方向:

1.酶的催化机制与构效关系解析

2.酶分子设计与定向进化

3.人工生物(多酶)合成途径与体外合成生物学催化体系的构建

4.结合生物催化与微生物合成生物学技术,高效合成手性药物、食品添加剂及高值化学品


代表性论文*通讯作者)

1. Wen Tang, Lulu Chen, Jian Deng, Yuyao Kuang, Chao Chen, Bo Yin, Hualei Wang, Jinping Lin*, Dongzhi Wei. Structure-guided evolution of carbonyl reductase for efficient biosynthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate. Catalysis Science & Technology. 2020, 10: 7512

2. Liandan Zheng, Jinping Lin*, Baoqi Zhang, Yuyao Kuang, Dongzhi Wei. Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)citronellal. Bioresources and Bioprocessing. 2018, 5:9

3. Xingxing Zou‡, Jinping Lin‡, Xinlei Mao, Shengyun Zhao,Yuhong Ren*. Biosynthesis of Lerythrose by assembly of two key enzymes in Gluconobacter oxydans. Journal of Agricultural and Food Chemistry. 2017, 65 (35), 7721

4. Kangling Chen, Kefei Li, Jian Deng, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol. Microbial Cell Factories. 2016, 15(1):191

5. Huan Zhang, Lulu Shi, Xinlei Mao, Jinping Lin*, Dongzhi Wei. Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003. Journal of Biotechnology. 2016, 237: 18

6. Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin*, Dongzhi Wei*. Towards the computational design and engineering of enzymeenantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans. Journal of biotechnology. 2016, 217: 31

7. Kefei Li, Xinlei Mao, Liu Liu, Jinping Lin*, Ming Sun, Dongzhi Wei and Shengli Yang. Overexpression of membranebound gluconate2dehydrogenase to enhance the production of 2ketodgluconic acid by Gluconobacter oxydans. Microbial Cell Factories. 2016, 15(1):121

8. Baoqi Zhang, Liandan Zheng, Jinping Lin*, Dongzhi Wei. Characterization of an ene-reductase from Meyerozyma guilliermondii for asymmetric bioreduction of α,β -unsaturated compounds. Biotechnology Letters. 2016, 38(9):1527

9. Yuanyuan Shi, Kefei Li, Jinping Lin*, Dongzhi Wei, Shengli Yang. Engineered expression vectors significantly enhanced the production of 2-keto-D-gluconic acid by Gluconobacter oxydans. Journal of Agricultural and Food Chemistry.2015, 63(22): 5492

10. Xingxing Zhang, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic. 2015, 112: 69

11. Rong Chen, Xu Liu, Jiale Wang, Jinping Lin*, Dongzhi Wei*. Cloning, expression, and characterization of an anti-Prelog stereospecic carbonyl reductase from Gluconobacter oxydans DSM2343. Enzyme and Microbial Technology. 2015, 70: 18

12. Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin, Dongzhi Wei*. Efficient synthesis of optically active halogenated aryl alcohols at highsubstrate load using a recombinant carbonyl reductase from Gluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic. 2015, 118: 1

13. Xu Liu, Rong Chen, Zhongwei Yang, Jiale Wang, Jinping Lin*, Dongzhi Wei. Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester. Molecular Biotechnology. 2014, 56: 285

14. Daihuan Li, Jinping Lin*, Dongzhi Wei. Improving Gluconobacter oxydans performance in the in situ removal of the inhibitor for asymmetric resolution of racemic 1-phenyl-1,2-ethanediol. Bioresource Technology. 2014, 159: 327

15. Lulu Shi, Kefei Li, Huan Zhang, Xu Liu, Jinping Lin*, Dongzhi Wei*. Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans. Journal of Biotechnology. 2014, 175:69

16. Xu Liu, Chao Wang, Lujia Zhang, Zhiqiang Yao, Dongbing Cui, Liang Wu, Jinping Lin* , Y. Adam Yuan, Dongzhi Wei. Structural and mutational studies on an aldo-keto reductase AKR5C3 from Gluconobacter oxydans. Protein science. 2014, 23(11):1540

17. Xu Liu, Zuanning Yuan, Y. Adam Yuan, Jinping Lin*, Dongzhi Wei. Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. Biochemical and Biophysical Research Communications. 2011,415: 410

18. Jian Wu, Minhua Li, Jinping Lin*, Dongzhi Wei. Highly Selective Oxidation of Benzyl Alcohol Using Engineered Gluconobacter Oxydans in Biphasic System. Current Microbiology. 2011, 62:1123

19. Jian Wu, Jiale Wang, Minhua Li, Jinping Lin*, Dongzhi Wei*. Optimization of immobilization for selective oxidation of  benzyl alcohol by Gluconobacter oxydans using response surface methodology. Bioresource Technology. 2010, 101:8936

20. Minghua Li, Jian Wu, Xu Liu, Jinping Lin*, Dongzhi Wei*, Hao Chen. Enchanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans. Bioresource Technology. 2010, 101:8294


专著教材

1.副主编:《酶工程》,高等教育出版社,2020.10ISBN 978-7-04-054779-5

2.参编:《生物催化剂与酶工程》,科学技术出版社,2008.09ISBN 978-7-03-022195-7


发明专利:

1.林金萍, 魏东芝,张行星。一种醇脱氢酶氧化二元伯醇的方法。ZL201310606518.5

2.林金萍, 魏东芝,张行星。一种应用微生物细胞催化法制备羟基醛的方法。 ZL201310601537.9

3.林金萍, 魏东芝,李克非。一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法。ZL201310598049.7

4.林金萍, 魏东芝,施露露。一种氧化葡萄糖酸杆菌启动子及其应用。ZL201310597667.X

5.林金萍, 魏东芝,杨圆圆。一种硝基还原酶在芳香族硝基化合物降解还原中国的应用。ZL201310627211.3

6.林金萍,魏东芝,吴建。一种同时分析微生物发酵液中甘油和二羟基丙酮的方法。ZL200910052444.9

7.林金萍, 魏东芝, 李明华, 吴建。一种氧化葡萄糖酸杆菌(G.oxydans)的基因工程菌及其应用。ZL20091096972.1

8.魏东芝,林金萍,高琼。一种微生物不对称拆分制备(S)-苯乙二醇的方法。ZL200810038899.0

9.魏东芝,林金萍,张敏洁。一种酶法连续生产聚(ε-己内酯)的方法。ZL 200910045661.5


获奖:

1.定向转化多元醇的生物催化剂创制及其应用关键技术。国家技术发明二等奖,2015

2.定向转化多元醇的细胞催化剂创制及其应用关键技术。上海市技术发明奖,一等奖,2013

3.手性非天然氨基酸生物催化制备技术及其应用。上海市科技进步奖,一等奖,2012

4.工业酶制剂的开发与应用关键技术。上海市科技进步奖,一等奖,2010


联系方式:

上海市梅陇路130311信箱 邮编:200237

办公室:鲁华生物技术研究所(实验14楼东楼)402

电话: 021-6425192313918729416

E-mail: jplin@ecust.edu.cn