吴辉
发布人:2020十大正规网赌网址网站管理员  发布时间:2016-10-19   动态浏览次数:15190

 


  


生物工程专业:博士生导师/硕士生导师

生物化工专业:博士生导师/硕士生导师

生物工程领域:工程硕士导师



吴辉,工学博士,副教授,博导。现任华东理工大学教务处副处长兼本科生招生办公室主任、中国轻工业生物基材料工程重点实验室副主任,中国化工学会生物化工专委会青年学者工作委员会委员,中国生物工程学会青年工作委员会委员,上海市生物工程学会合成生物学委员会委员,生物反应器工程国家重点实验室固定人员。教育部霍英东青年教师基金获得者(2018)。2019年被破格遴选为博士生导师。上海市生物工程学会东富龙生物工程优秀青年科学家奖(2020)。获中国石油与化工教育教学优秀成果一等奖(2020),华东理工大学教学成果一等奖(2021),华东理工大学教学成果二等奖(2019)。获20192020年度华东理工大学优秀研究生指导教师。

2004年毕业于南京工业大学生物工程系,获工学学士学位。2009年毕业于华东理工大学发酵工程专业,获工学博士学位。同年进入中科院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有南方的哈佛之称的美国莱斯大学生物工程系从事博士后研究工作。20149月引进全球十大网赌排行app,入选华东理工大学青年英才培育计划2018 A类、2015)等。作为负责人主持国家自然科学基金面上项目、青年项目、教育部霍英东青年教师基金、上海市自然科学基金等项目;作为主要研究骨干参与科技部国家重点研发计划课题、863计划等。作为负责人主持上海市教改项目2项:《微生物学(全英文)》入选上海市高校示范性全英语课程建设项目(2017)和《微生物学》入选上海市一流本科课程(2020。近年来在Metabolic EngineeringBiotechnology AdvancesACS Synthetic BiologyBiotechnology and BioengineeringBioresource TechnologyRedox Biology等本领域重点期刊上发表SCI收录论文40余篇。申请美国发明专利6项,已授权4项;申请中国发明专利12项,已授权8项;申请实用新型专利1 项。指导研究生多次获得校外奖学金和学术会议最佳墙报奖、华东理工大学第十三届华陆科技· 奋进杯大学生课外学术科技作品竞赛二等奖;共同指导并带队本科生获2017年和2018年连续两次获得国际遗传工程机器大赛(iGEM)竞赛金奖,指导大学生创新创业训练计划上海市级和校级项目。


研究方向:

1.微生物代谢工程生产平台化合物和生物活性物质

运用微生物代谢工程和合成生物学的最新技术和方法,合理设计及重构微生物代谢途径,结合精细化代谢调控策略,建立以可再生生物质资源(糖、甘油、乙酸、甲酸、CO2等)为原料的新型高效微生物细胞工厂,合成多种生物基化学品与生物基材料,如丁二酸,3-羟基丙酸,丙酮,异丙醇,生物聚酯,氨基酸,脂肪酸及其衍生物等。

2.合成生物学中代谢晶体管研究及其应用

代谢晶体管控制模式借鉴电子元器件晶体管的放大控制模式,通过精细调节细胞中微量关键物质的浓度,进而对整个细胞进行代谢重编程。

3 . 基于微生物电化学合成的代谢工程与合成生物学

运用代谢工程与合成生物学的策略,结合电化学平台技术与方法,搭建能利用一碳资源的自养型大肠杆菌细胞工厂平台,用于生产多种生物基化合物等。


主持和参加的科研项目:

1.主持国家自然科学基金面上基金 2018.1-2021.12

2.主持霍英东教育基金会第十六届高等院校青年教师基金 2018.3-2021.3

3.主持上海市自然科学基金 2019.6-2022.6

4.主持国家重点研发计划子课题2017.07 - 2021.06

5.主持中科院合成生物学重点实验室开放课题 2020.6-2022.6

6.主持国家自然科学基金青年科学基金 2015.1-2017.12

7.主持2020十大正规网赌网址优秀青年教师远志计划”2017.1-2018.12

8.主持工业生物催化教育部重点实验室开放课题 2015.12-2017.12

9.主持中央高校基本科研业务费探索研究基金  2015.7-2017.6

10.主持2020十大正规网赌网址优秀青年教师培育计划,2015.07-2017.07

11.参与上海市科技创新行动计划” 2013.9-2016.9

12.参与国家863计划基因组规模系统代谢育种” 2012.11-2015.12

13.参与国家863计划化学品生物转化过程与装备” 2012.11-2015.12


学术与社会兼职:

Frontiers in Microbiology, IF=5.64副主编2019-

Journal of Industrial Microbiology & Biotechnology, IF=3.346编委2021-

Bioresources and Bioprocessing, IF=4.578青年编委2020-

Bioresources and Bioprocessing, IF=4.578执行编辑2016-2019

Bioresources and Bioprocessing, IF=4.578客座主编2015-2016

Frontiers in Bioengineering and Biotechnology, IF=5.89编委2016-

Frontiers in Bioengineering and Biotechnology, IF=5.89客座副主编2019,2020

美国微生物学会ASM会员2015-

美国工业微生物与生物技术学会SIMB会员2013-

中国化工学会生物化工专委会青年学者工作委员会委员(2017-2021

中国生物工程学会青年工作委员会委员(2019-2023

上海市生物工程学会合成生物学委员会委员(2018-2022

多个国际杂志如Biotechnology AdvancesMetabolic EngineeringBiotechnology and BioengineeringBiotechnology for BiofuelScientific ReportsMicrobial Cell FactoriesFrontiers in MicrobiologyJournal of Agricultural and Food ChemistryBioresource TechnologyBiotechnology JournalBiochemical Engineering JournalProcess BiochemistryBioprocess and Biosystems EngineeringBioresources and BioprocessingFrontiers in Bioengineering and BiotechnologyApplied Biochemistry and BiotechnologyBiotechnology and Applied Biochemistry等审稿人。多个国内杂志《生物工程学报》、《微生物学报》、《生物加工过程》、《微生物学报》等期刊审稿人。


本科和研究生教学:

1. 本科专业必修课,《微生物(全英文)》,48学时,3学分;

2. 博士研究生专业课程,《系统代谢工程》(与花强教授合作),32学时,2学分;

3. iGEM竞赛导师,Bio-maker创新活动指导老师,大创指导老师。


代表性论文(*通讯作者):  

1. Xiangju Wei, Ju Wu, Pengye Guo, Shengmin Zhou, Hui Wu*. Effect of short-chain thioesterase deficiency on p(3HB-co-LA) biosynthesis in Escherichia coli. Chinese Journal of Biotechnology. 2021. 37(1):196-206.

2. Guohui Li, Dixuan Huang, Xue Sui, Shiyun Li, Bing Huang, Xiaojuan Zhang*, Hui Wu*, Yu Deng*. Advances in microbial production of medium-chain dicarboxylic acids for nylon materials. Reaction Chemistry & Engineering. 2020. 5:221-238 (IF=4.239, JCR Q2)

3. Hao Yang, Can Zhang, Ningyu Lai, Bing Huang, Peng Fei, Dawei Ding, Peng Hu, Yang Gu, Hui Wu*. Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation. Bioresource Technology. 2020. 296:122337. (IF=9.642, JCR Q1)

4Qiaofei He, George N. Bennett, Ka-Yiu San, Hui Wu*.Biosynthesis of medium-chain ω-hydroxy fatty acids by AlkBGT of Pseudomonas putida GPo1 with native FadL in engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology. 2019. 7:273. (IF=5.89, JCR Q1)

5Hao Yang#, Bing Huang#, Ningyu Lai, Yang Gu, Zhimin Li, Qin Ye, Hui Wu*. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories.2019. 18:6. (IF=5.328, JCR Q1)

6. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology.2019. 35(1):59-69.

7Jiapeng Tang, Zhenqing Qian, Hui Wu*. Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source. Bioresource Technology. 2018. 268:60-67. (IF=9.642, JCR Q1)

8Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 2018. 115:943-954. (*Co-corresponding) (IF=4.53, JCR Q2)

9Wei Li#, Hui Wu#, Mai Li, Ka-Yiu San*. Effect of NADPH availability on free fatty acid production in E. coli. Biotechnology and Bioengineering. 2018. 115:444-452. (#First two authors contributed to this paper equally) (IF=4.53, JCR Q2)

10Han Liu, Guochen Fang, Hui Wu*, Zhimin Li*, Qin Ye. L-cysteine production in Escherichia coli based on rational metabolic engineering and modular strategy. Biotechnology Journal. 2018. 13:e1700695. (*Co-corresponding) (IF=4.677, JCR Q1)

11Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation. Bioresources and Bioprocessing. 2018. 5:41. (Feature Article) (IF=4.578, JCR Q2)

12. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing. 2018. 5:34. (*Co-corresponding) (IF=4.578, JCR Q2)

13Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 2017. 231:75-84. (IF=9.642, JCR Q1)

14Xing Zhang#, Hui Wu#, Bing Huang, Zhimin Li*, Qin Ye. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 2017. 241:163-169. (# First two authors contributed to this paper equally) (IF=3.50, JCR Q2)

15Qing Li#, Hui Wu#, Zhimin Li, Qin Ye*. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 2016. 218:217-223. (# First two authors contributed to this paper equally) (IF=9.642, JCR Q1)

16Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 2016. 5:1299-1307. (*Co-corresponding) (IF=5.11, JCR Q1)

17Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 2016. 100(14):6279-6289. (*Co-corresponding) (IF=4.813, JCR Q1)

18Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 2016. 15:38. (*Co-corresponding) (IF=5.328, JCR Q1)

19Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 2016. 43:45-53. (*Co-corresponding) (IF=3.346, JCR Q2)

20Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 2015. 214:63-68. (*Co-corresponding). (IF=3.50, JCR Q2)

21Hui Wu, Leepika Tuli, George N. Bennett, Ka-Yiu San*. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli. Metabolic Engineering. 2015. 28:159-168. (IF=9.783, JCR Q1)

22Hui Wu, George N. Bennett, Ka-Yiu San*. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering. 2015. 112(8):1720-1726. (IF=4.53, JCR Q2)

23Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 2015. 185:431-435. (IF=9.642, JCR Q1)

24Hui Wu, Mukund Karanjikar, Ka-Yiu San*. Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol. Metabolic Engineering. 2014. 25:82-91. (IF=9.783, JCR Q1)

25Hui Wu, Ka-Yiu San*. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 2014. 111(11):2209-2219. (IF=4.53, JCR Q2)

26Yane Luo*, Tao Zhang, Hui Wu*. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 2014. 32:905-919. (IF=14.227, JCR Q1)

27Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Efficient free fatty acid production from woody biomass hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 2014. 169:119-125. (IF=9.642, JCR Q1)

28Hui Wu, Ka-Yiu San*. Engineering Escherichia coli for odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology.2014. 98(19):8145-8154. (IF=4.813, JCR Q1)

29Hui Wu, Qing Li, Zhi-min Li*, Qin Ye*. Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 2012. 107:376-384. (IF=9.642, JCR Q1)

30Yuan Liu, Hui Wu, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology.2011. 49(5):459-64. ( First two authors contributed to this paper equally) (IF=3.493, JCR Q2)

31Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 2009. 44:165-169. (IF=3.493, JCR Q2)

32Hui Wu, Zhi-min Li, Li Zhou, Qin Ye*. Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology. 2007. 73:7837-7843. (IF=4.792, JCR Q1)


其他合作发表文章:

1. Haitao Deng, Jingyi Li, Yao Zhou, Yang Xia, Chao Chen, Zhemin Zhou, Hui Wu, Ping Wang, Shengmin Zhou*. Genetic engineering of circularly permuted yellow fluorescent protein reveals intracellular acidification in response to nitric oxide stimuli, Redox Biology, 2021. 41: 101943. (IF=11.799, JCR Q1)

2. Haritha Meruvu, Hui Wu, Ziyue Jiao, Liyan Wang, Qiang Fei*, From Nature to Nurture: Essence and methods to isolate robust methanotrophic bacteria, Synthetic and Systems Biotechnology, 2020. 5(3):173-178.(IF=4.708, JCR Q1)

3Yunpeng Yang#, Nannan Lang#, Lu Zhang, Hui Wu, Weihong Jiang*, Gu Yang*. A novel regulatory pathway consisting of a two-component system and an ABC-2 type transporter controls butanol tolerance in Clostridium acetobutylicum. Applied Microbiology and Biotechnology. 2020. 104(11):5011-5023(IF=4.813, JCR Q2)

4Jiawei Li, Xiaoyan Zhang, Hui Wu, Yunpeng Bai*. Transcription factor screening and engineering for metabolic engineering and production of organic acids: an overview. Frontiers in Bioengineering and Biotechnology. 2020. 8:98 (IF=5.89, JCR Q1)

5Boliang Gao, Li Li, Hui Wu, Du Zhu, Min Jin, Wu Qu, Runying Zeng*. A novel strategy for efficient agaro-oligosaccharide production based on the enzymatic degradation of crude agarose in Flammeovirga pacifica WPAGA1. Frontiers in Microbiology. 2019. 10:1231. (IF=5.64,JCR Q1)

6Xiangwei Cui, Junxian Wan, Xing Zhang, Hui Wu, Zhimin Li*, Qin Ye. Efficient glutathione production in metabolically engineered Escherichia coli strains using constitutive promoters. Journal of Biotechnology. 2019. 289:39-45 (IF=3.307, JCR Q2)

7Bing Huang, Guocheng Fang, Hui Wu, Jibin Sun, Zhimin Li*, Qin Ye. Efficient biosynthesis of succinate from paper mill wastewater by engineered Escherichia coli. Applied Biochemistry and Biotechnology. 2019. 189:1195-1208. (IF=2.92, JCR Q3)

8.  Feng Li#, Yuanxiu Li#, Liming Sun, Xiaoli Chen, Xingjuan An, Changji Yin, Yingxiu Cao,Hui Wu, Hao Song*. Modular engineering intracellular NADH regeneration boosts extracellular electron transfer of Shewanella oneidensis MR-1. ACS Synthetic Biology. 2018. 7(3):885-895. (IF=5.11, JCR Q1)

9Zhuan Cheng, Jiaqi Jiang, Hui Wu, Zhimin Li*, Qin Ye. Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology. 2016. 200:897-904. (IF=9.642, JCR Q1)

10Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N Bennett, Ka-Yiu San*. Efficient free fatty acid production in engineered Escherichia coli strains using soybean oligosaccharides as feedstock. Biotechnology Progress. 2015. 31:686-694. (IF=2.681, JCR Q2)

11Yang Gu, Yu Jiang,Hui Wu, Xudong Liu, Zhilin Li, Jian Li, Han Xiao, Zhaobing Shen, Hongjun Dong, Yunliu Yang, Yin Li, Weihong Jiang*, Sheng Yang*. Economical challenges to microbial producers of butanol: feedstock, butanol ratio and titer. Biotechnology Journal. 2011. 6(11):1348-1357. (IF=4.677, JCR Q1)

 

撰写著作: 

1. Qiang Fei, Haritha Meruvu, Hui Wu, Rongzhan FuChapter 12: Biological Conversion of Natural Gas in Direct Natural Gas Conversion to Value-Added Chemicals, 512 Pages, ISBN 9780367077938, 2020, CRC Press.

2.  Qin Ye*, Zhimin Li, Hui Wu. Principle and performance of gas self-inducing reactors and applications to biotechnology. Bioreactor Engineering Research and Industrial Applications II, Advances in Biochemical Engineering/Biotechnology, Volume 152, 2016. ISBN: 978-3-662-48346-6 (Print), 978-3-662-48347-3 (eBook). Springer, Berlin, Heidelberg, Germany.


发明专利:

1Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis. US Patent Number: 10851393B2. 授权.

2Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 10,011,839 B2. 授权.

3Ka-Yiu San, Xixian Xie, Leepika Tuli,Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes. US patent Number: 9,487,804 B2. 授权.

4Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253 B2. 授权.

5Ka-Yiu San and Hui Wu. Integrated biodiesel process. US patent. Publication Number: 2014/0335578 A1.

6Ka-Yiu San and Hui Wu. Microbial odd chain fatty acids. US patent. Publication Number: 2014/0193867 A1.

7吴辉,陆静娴,李志敏,叶勤. 一种提高大肠杆菌合成的聚(3-羟基丁酸-co-乳酸)中乳酸组分含量的方法.中国发明专利专利号:ZL201810246568.X.

8吴辉,杨昊,李志敏,黄兵,叶勤. 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利专利号:ZL201710978174.9. 授权.

9吴辉,黄兵,李志敏,杨昊,叶勤. 利用乙酸生产羟基丙酸的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利专利号:ZL201710969403.0. 授权.

10叶勤,李晴,李志敏吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. 中国发明专利专利号:ZL201610305322.6. 授权.

11李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. 中国发明专利专利号:ZL201410599042.1. 授权.

12周通,刘映淼,孙兵兵,王祎,吴辉,杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法. 中国发明专利专利号:ZL201010598350. 授权.

13叶勤李志敏吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. 中国发明专利专利号:ZL200810207564. 授权.

14吴辉李志敏叶勤. 大肠杆菌生产琥珀酸的方法. 中国发明专利专利号:ZL200710135684. 授权.

15. 吴辉,费鹏,赖宁玉. 利用乙酸或乙酸盐生产3-羟基丁酸的代谢工程大肠杆菌菌株的构建方法及应用.中国发明专利专利申请号: 202011464753.X.

16. 吴辉,魏香菊,吴榉,郭鹏业. 一种提高聚羟基丁酸乳酸酯中乳酸组分含量的基因工程大肠杆菌的构建方法及应用.中国发明专利专利申请号:202010214416.9.

17. 吴辉,赖宁玉,赵舒欣,王文卓,黎冠忠. 利用乙酸生产3-羟基丙酸的代谢工程大肠杆菌菌株的构建方法及应用.中国发明专利专利申请号:201910928013.8.

18. 陆健,谭高翼,王启要,吴辉,夏建业,李友元,李晔,李媛媛. 一种用于光合生物反应器的发光搅拌桨. 专利公开(公告)号:CN207646198U

19李志敏, 黄兵, 吴辉, 房国辰, 叶勤.一种利用造纸废液生物合成丁二酸的方法. 中国发明专利专利申请号: 201711142577.6.

 

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E-mail: hwu@ecust.edu.cn