王风清
发布人:2020十大正规网赌网址网站管理员  发布时间:2016-10-19   动态浏览次数:11850


王风清

生物化工专业:硕士生导师

生物化学与分子生物学专业:博士生导师/硕士生导师 

  

   

2000年和2003年分别于天津科技大学获学士和硕士学位,2007年毕业于华东理工大学,获生物化工博士学位。长期致力于“微生物代谢工程”相关的研究工作,研究内容涉及:“甾体药物微生物代谢转化合成”、“草本天然产物的微生物合成”、“脂质转化及药物修饰用高值酶”等。近几年,以第一作者或通讯作者在Metabolic engineering”、Biomaterials”、Environmental Science & Technology Applied and Environmental Microbiology等本领域重点期刊上发表SCI论文多篇。

研究方向:

利用代谢工程和合成生物学的原理和方法,致力于微生物细胞工厂的研究和开发,主要研究内容分:一、针对工业微生物生产领域中的一些关键瓶颈问题,开展生理生化、组学、代谢工程和合成生物学研究,开发高效能微生物菌种。涉及的品种:甾体药物、抗生素和营养补充剂等。二、   针对动植物来源的高值活性天然产物和低产但高附加值的微生物次生代谢产物,开展微生物异源全合成研究。涉及品种:甾体型天然产物、中药活性天然产物、萜类香料和微生物次生代谢产物等。三、针对肠道微生物,开展疾病探测、给药和肠道生态工程化干预等工作。

教育经历:

2003-2007   华东理工大学,生物工程学院,博士

2000-2003   天津科技大学,生物工程学院,硕士

1996-2000   天津科技大学,食品工程系,学士

承担项目:

2019-2029   企业横向课题  甾体生物发酵新菌种与技术

2018-2021   国家自然科学基金面上项目  仿动物甾体激素代谢模式创建甾体药物合成微生物细胞工厂的研究(No. 21776075

2014-2017   国家自然科学基金面上项目  在分枝杆菌中从简单碳源自给自足合成雄甾烯酮的研究 (No. 31370080)

2013-2015   国家自然科学基金青年项目  细胞壁在Mycolata 类微生物摄取转化甾醇过程中的作用 (No. 21206039)

2013-2014   企业横向课题  柠檬酸菌株基因工程改造

2011-2013   中央高校基本科研业务费  甾醇侧链C17-C20键断裂的机理及其基因调控在甾体药物生产过程中的作用

2011-2012   863项目子课题  柠檬酸、葡萄糖酸的生物转化技术   柠檬酸高品质生产菌株的研发

2008-2010   自然基金项目  具有新颖催化特征的S. virginiae新菌株降解薯蓣皂素、雌酮等甾类内分泌干扰物的新机制研究 (No. 20777016)

2008-2010   863项目   基于类固醇药物生产相关重要生物转化反应的工程微生物的研发 (NO. 2008AA02Z209)

产业化项目:

微生物转化甾醇制备甾体医药中间体。

磷脂改性高值产物。

代表论文(* 通讯作者)

1. Guo-Song Liu, Tian Li, Wei Zhou, Min Jiang, Xin-Yi Tao, Min Liu, Ming Zhao, Yu-Hong Ren, Bei Gao*, Feng-Qing Wang*, Dong-ZhiWei*. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction. Metabolic Engineering, 2020. DOI: 10.1016/j.ymben.2019.11.001

2. Wan-Ju Sun, Li Wang, Hao-Hao Liu, Yong-Jun Liu, Yu-Hong Ren, Feng-Qing Wang*, Dong-Zhi Wei. Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum. Metabolic Engineering, 2019. 56: 97-110.

3. Bin Ma, Min Liu, Zhen-Hai Li, Xin-Yi Tao, Dong-Zhi Wei, Feng-Qing Wang*. Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae. Journal of Agricultural and Food Chemistry, 2019, 67(31), 8590-8598.

4. Min Liu, Liang-Bin Xiong, Xinyi Tao, Qing-Hai Liu, Feng-Qing Wang*, Dong-Zhi Wei*. Metabolic adaptation of Mycobacterium neoaurum ATCC 25795 in the catabolism of sterols for producing important steroid intermediates. Journal of Agricultural and Food Chemistry. 2018, 66(45)12141-12150.

5. Min Liu; Liang-Bin Xiong; Xinyi Tao; Qing-Hai Liu; Feng-Qing Wang*; Dong-Zhi Wei*. Integrated transcriptome and proteome studies reveal the underlying mechanisms for sterol catabolism and steroid production in Mycobacterium neoaurum. Journal of Agricultural and Food Chemistry. 2018, 66 (34): 9147-9157.

6. Hao-Hao Liu, Li-Qin Xu, Kang Yao, Liang-Bin Xiong, Xin-Yi Tao, Min Liu, Feng-Qing Wang*, Dong-Zhi Wei. Engineered 3-ketosteroid 9α-hydroxylases in Mycobacterium neoaurum: an efficient platform for production of steroid drugs. 2018, Applied and Environmental Microbiology, 2018 84(14):e02777-17.

7. Liang-Bin Xiong, Wan-Ju Sun, Yong-Jun Liu, Feng-Qing Wang*, Dong-Zhi Wei. Ehancement of 9α-hydroxy-4-androstene-3,17-dione production from soybean phytosterols by deficiency of a regulated intramembrane proteolysis metalloprotease in Mycobacterium neoaurum. Journal of Agricultural and Food Chemistry. 2017, 65(48), 10520-10525.

8. Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Wan-Ju Sun, Feng-Qing Wang*, Dong-Zhi Wei*. Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes. Microbial Cell Factories. 2017, 16:89-.

9. Xinyi Tao, Ning Jia, Nenghui Cheng, Yuhong Ren, Xuni Cao, Min Liu, Dongzhi Wei*, Feng-Qing Wang*. Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug. Biomaterials. 2017, 131:1-14.

10. Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Dong-Zhi Wei, Feng-Qing Wang*. Role identification and application of SigD in the transformation of soybean phytosterol to 9α-hydroxy-4-androstene-3,17-dione in Mycobacterium neoaurum. Journal of Agricultural and Food Chemistry. 2017, 65(3):626-631.

11. Zhongwei Yang, Xin Gao, Hui Xie, Fengqing Wang, Yuhong Ren, Dongzhi Wei*. Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli. Biotechnology and Bioengineering. 2017, 114:457-462

12. Min Liu, Zhan-Tao Zhu, Xin-Yi Tao, Feng-Qing Wang*, Dong-Zhi Wei. RNA-seq analysis uncovers non-coding small RNA system of Mycobacterium neoaurum in the metabolism of sterols to accumulate steroid intermediates. Microbial cell Factories. 2016, 15:1-17.

13. Zhao CGao XLiu XWang YYang SWang FQ*Ren Y*. Enhancing Biosynthesis of a Ginsenoside Precursor by Self-assembly of Two Key Enzymes in Pichia pastoris. Joural of agriculture and food chemistry. 2016, 64:3380-3385.

14. Li-Qin Xu, Yong-Jun Liu, Kang Yao, Hao-Hao Liu, Xin-Yi Tao, Feng-Qing Wang*, Dong-Zhi Wei. Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism. Scientific Reports, 2016, 6:21928.

15. Xin-Bin Liu, Min Liu, Xin-Yi Tao, Zhong-Xi Zhang, Feng-Qing Wang*, Dong-Zhi Wei. Metabolic engineering of Pichia pastoris for the production of dammarenediol-II. Journal of biotechnology, 2015, 216: 47-55.

16. Kang Yao, Li-Qin Xu, Feng-Qing Wang*, Dong-Zhi Wei. Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols. Metabolic Engineering, 2014, 24: 181-191.

17. Kang Yao, Feng-Qing Wang*, Huai-Cheng Zhang, Dong-Zhi Wei. Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum. Metabolic Engineering, 2013, 15:75-87.

联系方式:

上海市梅陇路130311信箱 邮编:200237
办公室:鲁华生物技术研究所401

021-64253287, 13816715931

E-mail: fqwang@ecust.edu.cn

https://www.researchgate.net/profile/Feng_Qing_Wang