周燕
发布人:2020十大正规网赌网址网站管理员  发布时间:2016-10-19   动态浏览次数:8765

 

周燕

  

生物化工专业:博士生导师/硕士生导师

生物工程与技术专业:博士生导师/硕士生导师

生物与医药工程专业:工程硕士生导师

  

个人介绍

教授,博士生导师。

1988年获华东化工学院(现华东理工大学)生物化学专业理学学士学位;1991年获华东理工大学生物化工专业工学硕士学位;毕业后留校任教,期间曾在香港城市大学生物及化学系开展研究工作。现任职华东理工大学生物反应器工程国家重点实验室动物细胞与组织工程研究团队,从事动物细胞培养技术研究及其在干细胞/成体细胞体外大规模培养和人体组织体外构建中的应用。承担并完成国家级和省部级科研项目二十余项,在国内外核心刊物发表论文一百二十余篇,申请、公开或授权国家发明专利十多项,获上海市科技进步奖二等奖。


研究方向:

1.体外培养环境调控干细胞扩增和分化的机制

2.干细胞体外规模化扩增和定向分化诱导技术

3.工程化人体组织体外构建新技术及生物反应器研制

4.动物细胞体外培养的无血清培养基设计和开发

5.动物细胞培养过程优化及抗体、病毒疫苗高效生产技术


主持或参加的科研项目或基金:

1.国家重大研发计划:生物力学调控细胞生物学行为及微组织构建

2.国家“863”计划:(1)组织工程种子细胞体外大量扩增技术研究;(2)临床级干细胞产业化技术研发;(3)人体皮肤组织工程研究

3.国家重大新药创制:哺乳动物细胞大规模培养及药物制备关键技术

4.国家支撑计划:动物细胞大规模无血清培养及表达产品制备技术

5.国家自然科学基金:(1)微组织体外构建技术及其过程分析;(2)基于微构重塑技术构建大型预血管化工程组织

6.上海市重大科研攻关:骨髓间充质干细胞体外扩增及成骨诱导的应用研究

7.上海市基础研究重点:(1)hiHep体外规模化扩增;(2)功能性聚酯基组织工程材料制备及其对组织再生调控的研究

8.上海市科技兴农重点攻关:(1)微载体技术用于兽用细胞疫苗产业化研究;(2)猪流行性腹泻、猪传染性胃肠炎和轮状病毒的诊断及三联苗研制


教学:

1.生物技术专业本科生:《细胞工程》

2.生物工程学院研究生:《细胞培养工程》


代表性论文(*通讯作者):

1.Jiazhu Xu, Qi Fang, Yanli Liu, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. In situ ornamenting poly(ε-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells. Colloids and Surfaces B-Biointerfaces. 2021; 197: 111374.

2.Chao Wei#, Bingyu Liang#, Yongsheng Li#, Bingkun Yan, Jiaqian Hou, Yan Zhou*, Yingbin Liu*, Meidong Lang*. A drug-free therapeutic system for cancer therapy by diselenide-based polymers themselves. Advanced Healthcare Materials. 2021; 10(2): e2001471.

3.Miaomiao Chai, Ce Gu, Qihua Shen, Jiaxing Liu, Yi Zhou, Ziyang Jin, Wanli Xiong, Yan Zhou*, Wensong Tan. Hypoxia alleviates dexamethasone-induced inhibition on angiogenesis in co-culture of HUVECs and rBMSCs via HIF-1α. Stem Cell Research & Therapy. 2020; 11(1): 343.

4.Wei Liu, Mi Zhang, Miaomiao Zhou, Ce Gu, Zhaoyang Ye, Yan Xiao*, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and evaluation of modified poly(ethylene terephthalate) microfibrous scaffolds for hepatocyte growth and functionality maintenance. Materials Science & Engineering C-Materials for Biological Applications. 2020; 109: 110523.

5.Wei Liu, Mi Zhang, Yan Xiao, Zhaoyang Ye, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds. Biochemical Engineering Journal. 2020; 160: 107565.

6.Ce Gu, Miaomiao Chai, Jiaxing Liu, Hui Wang, Wenjing Du, Yan Zhou*, Wen-Song Tan. Expansion of transdifferentiated hepatocyte in a serum-free microcarrier culture system. Digestive Diseases and Sciences. 2020; 65(7): 2009-2023.

7.Jiaxing Liu, Hui Wang, Wenxia Ren, Yan Zhou*, Wen-Song Tan. β-mercaptoethanol promotes osteogenesis of human mesenchymal stem cells via sirt1-ERK pathway. Cytotechnology. 2020; 72(5): 695-706.

8.Qihua Shen, Xiaoting Fan, Mingli Jiang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture. Archives of Biochemistry and Biophysics. 2019; 675: 108108.

9.Huimin He, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask. Cell Proliferation. 2019; 2019: e12587. 

10.Ce Gu, Panpan Li, Wei Liu, Yan Zhou*, Wen-Song Tan. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. Journal of Cellular and Molecular Medicine. 2019; 23(6): 4165-4178.

11.Jie Sun, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. Transforming growth factor-β1 stimulates mesenchymal stem cell proliferation by altering cell cycle through the FAK-Akt-mTOR pathway. Connective Tissue Research. 2019; 60(4): 406-417.

12.Anmin Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Bioactive Poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration. Journal of Biomaterials Applications. 2019; 33(9): 1242-1251.

13.Wei Liu, Dan Hu, Ce Gu, Yan Zhou*, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on an optimum two-stage culture strategy. Journal of Bioscience and Bioengineering. 2019; 127(4): 506-514.

14.Pan-Pan Li, Ce Gu, Bing-Yu Liang, Lin Wang, Yan Zhou*, Wen-Song Tan. A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes. Cytotechnology. 2019; 71(1): 329-344.

15.Yan Xiao*, Miaomiao Zhou, Mi Zhang, Wei Liu, Yan Zhou*, Meidong Lang*. Hepatocyte culture on 3D porous scaffolds of PCL/PMCL. Colloids and Surfaces B-Biointerfaces. 2019; 173: 185-193.

16.Mengjuan Liu, Jiaqi Wang, Hongping Tang, Li Fan, Liang Zhao, Hai-Bin Wang, Yan Zhou*, Wen-Song Tan. Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody. Biotechnology Letters. 2018; 40(11-12): 1487-1493.

17.Xiaoting Fan, Yi Teng, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs. Journal of Cell Science. 2018; 131(13): jcs216135. DOI: 10.1242/ jcs.216135.

18.Fan Xiaoting, Li Lei, Ye Zhaoyang*, Zhou Yan*, Tan Wensong. Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate. Cell Biology International. 2018; 42(4): 457-469.

19.Liu Yanli, Xu Jiazhu, Zhou Yan, Ye Zhaoyang*, Tan Wensong. Layer-by-layer assembled polyelectrolytes on honeycomb-like porous poly(ε-caprolactone) films modulate the spatial distribution of mesenchymal stem cells. Materials Science & Engineering: C-Materials for Biological Applications. 2017; 78: 579-588.

20.Zhang Songjie, Zhou Min, Ye Zhaoyang*, Zhou Yan*, Tan Wensong. Fabrication of viable and functional pre-vascularized modular bone tissues by coculturing MSCs and HUVECs on microcarriers in spinner flasks. Biotechnology Journal. 2017; 12(8): 1700008. 

21.Chen Min, Xu Lei, Zhou Yan, Zhang Yan, Lang Meidong, Ye Zhaoyang*, Tan Wensong*. Poly(ε-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis. Cell Proliferation. 2016; 49(4): 512-522.

22.Panpan Xie, Huijie Niu, Xinning Chen, Xintao Zhang, Shiwei Miao, Xiancun Deng, Xuping Liu, Wen-Song Tan, Yan Zhou*, Li Fan*. Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures. Applied Microbiology and Biotechnology. 2016; 100(24): 10343-10353.

23.Lei Xu, Yuxi Wu, Zhimiao Xiong, Yan Zhou, Zhaoyang Ye*, Wen-Song Tan*. Mesenchymal Stem Cells Reshape and Provoke Proliferation of Articular Chondrocytes by Paracrine Secretion. Scientific Reports. 2016; 6: 32705.

24.Ludi Zhang, Yanjiao Shao, Lu Li, Feng Tian, Jin Cen, Xiaotao Chen, Dan Hu, Yan Zhou, Weifen Xie, Yunwen Zheng, Yuan Ji, Mingyao Liu, Dali Li*, Lijian Hui*. Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I. Scientific Reports. 2016; 6: 31460.

25.Xiao-Lei Shi, Yimeng Gao, Yupeng Yan, Hucheng Ma, Lulu Sun, Pengyu Huang, Xuan Ni, Ludi Zhang, Xin Zhao, Haozhen Ren, Dan Hu, Yan Zhou, Feng Tian, Yuan Ji, Xin Cheng, Guoyu Pan*, Yi-Tao Ding*, Lijian Hui*. Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes. Cell Research. 2016; 26(2): 206-216.

26.Yingdi Zhao, Ke Tan, Yan Zhou, Zhaoyang Ye*, Wen-Song Tan. A combinatorial variation in surface chemistry and pore size of three-dimensional porous poly(ϵ-caprolactone) scaffolds modulates the behaviors of mesenchymal stem cells. Materials Science and Engineering: C-Materials for Biological Applications. 2016; 1(59): 193-202.

27.Yaya Liu, Weiyan Zhang, Xiancun Deng, Hong Fai Poon, Xuping Liu, Wen-Song Tan, Yan Zhou, Li Fan*. Chinese hamster ovary cell performance enhanced by a rational divide-and conquer strategy for chemically defined medium development. Journal of Bioscience and Bioengineering. 2015; 120(6): 690-696.

28.Min Chen, Yi Zhang, Yan Zhou, Yan Zhang, Meidong Lang, Zhaoyang Ye*, Wensong Tan*. Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells. Colloids and Surfaces B Biointerfaces. 2015; 134: 322-331.

29.Houyong Luo, Maiqin Chen, Xiu Wang, Yang Mei, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Fabrication of centimeter-sized viable 3D tissue constructs endowed with microchannel conduits for improved tissue properties through assembling of cell-laden microbeads. Journal of Tissue Engineering and Regenerative Medicine. 2014; 8: 493-504.

30.Xiu Wang, Qingling Jiao, Songjie Zhang, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Perfusion culture induced template-assisted assembling of cell-laden microcarriers is a promising route for fabricating macrotissues. Biotechnology Journal. 2014; 9: 1425-1434.

31.Maiqin Chen, Min Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan*. Ectopic osteogenesis of macroscopic tissue constructs assembled from human mesenchymal stem cell-laden microcarriers through In vitro perfusion culture. PLoS ONE. 2014; 9(10): e109214.

32.Wei Wu, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells. The International Journal of Artificial Organs. 2011; 34(12): 1128-1136.

33.Maiqin Chen, Xiu Wang, Zhaoyang Ye, Yan Zhang, Yan Zhou*, Wen-Song Tan*. A modular approach to the engineering of centimeter-sized bone tissue constructs using human amniotic mesenchymal stem cells-laden microcarriers. Biomaterials. 2011; 32(30): 7532-7542.

34.Min Wang, Yan Zhou*, Wen-Song Tan. Clonal isolation and characterization of mesenchymal stem cells from human amnion, Biotechnology and Bioprocess Engineering. 2010; 15(6): 1047-1058.

35.Yang Mei, Qiang Tang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering, Journal of Biotechnology. 2010; 150(3): 438-446.


发明专利:

1.周燕,张咪,刘伟,谭文松 一种用于肝细胞三维培养的生物支架及其制备方法和应用

2.周燕,梁冰玉,杨晨曦,李盼盼,叶朝阳,谭文松 适于肝样细胞体外培养的替代血清组合物及其使用方法

3.周燕,吴伟,谭文松,用于骨髓间充质干细胞体外培养和扩增的无血清培养基

4.谭文松,赵亮,周燕,黄锭 适于MDCK细胞大规模贴壁培养和单细胞悬浮培养的无血清培养基

5.谭文松,赵亮,范里,刘旭平,周燕,孙亚婷 适于动物细胞高密度培养和产物表达的营养物添加剂

6.牛红星,赵亮,范里,周燕,谭文松,适用于NS0细胞大规模培养及抗体生产的无蛋白培养基



联系方式:

电话:021-64251570

传真:021-64252250

邮箱:zhouyan@ecust.edu.cn

地址:上海市梅陇路130号华东理工大学实验十八楼1013室

信箱:华东理工大学309信箱,邮编200237